Adipose tissue-derived stem cell expansion on collagen/chitosan scaffolds in bioreactor

clinical grade human adipose tissue-derived mesenchymal stem cell banking

in this study, our aim was to produce a generation of gmp-grade adipose tissue-derived mesenchymal stem cells for clinical applications. according to our results, we fulfill to establish consistent and also reproducible current good manufacturing practice (cgmp) compliant adipose tissue-derived mesenchymal stem cells from five female donors. the isolated cells were cultured in dmem supplemented...

Cytotoxicity of Chitosan Derived from Shrimp for Bone Scaffold on Adipose Tissue-Derived Mesenchymal Stem Cells

Expansion of mesenchymal adipose-tissue derived stem cells in a stirred single-use bioreactor under low-serum conditions

Materials and methods Preliminary tests in disposable-spinners (100 mL culture volume, Corning) were used to determine two suitable media and MC-types for serum reduced expansions (< 10%) of human adipose tissue-derived stem cells (hADSCs; passage 2, Lonza). Using such optimized media-MC-combinations, hADSCs expanded 30 to 40fold, which compares well with expansion rates in planar culture. Base...

P70: Produce Neural Stem Cell from Neurosphere of Rat Adipose Derived Stem Cell

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The effect of mesenchymal stem cell supernatant product derived from adipose tissue on sperm quality increase after defecation

Background & Goal: Infertility and the resulting individual and social problems are one of the main and most important issues and concerns of couples, and about half of all infertility is related to men. In the present study of mesenchymal stem cell supernatant products derived from Adipose tissue has been used as a preservative and sperm damage after defecation has been evaluated. Material & m...